Journal: Genome Biology
Article Title: Primary and secondary transcriptional effects in the developing human Down syndrome brain and heart
doi: 10.1186/gb-2005-6-13-r107
Figure Lengend Snippet: Increased transcript levels of genes assigned to chromosome 21 in TS21 samples compared to controls. The plots show ratio (TS21/euploid) of mean expression values, calculated using data from samples in each tissue or cell type, for all 23 chromosomes. (X and Y chromosome data were pooled.) The expression values were obtained with Affymetrix MAS5 software. The error bars represent standard errors (obtained by performing 1,000 iterations of a bootstrap resampling of the tissues). ( a ) The ratio of TS21 to euploid mean expression values for each chromosome in fetal cerebrum samples. ( b ) The ratio of TS21 to euploid mean expression values in fetal cerebellum samples. ( c ) The ratio of TS21 to euploid mean expression values in cultured astrocyte cell lines derived from fetal cerebrum tissues. ( d ) The ratio of TS21 to euploid mean expression values in fetal heart samples. ( e ) The ratio of TS21 to euploid mean expression values using data from all the above tissue and cell types.
Article Snippet: Raw data from U133A GeneChips were processed using both Affymetrix Microarray Suite version 5.0 (MAS5) software and robust multi-chip analysis (RMA) normalization (R version 1.7.1) from BioConductor [ ].
Techniques: Expressing, Software, Cell Culture, Derivative Assay